He aha te whakamahinga o te Polyacrylamide mō te electrophoresis pūmua

I roto i te ao o te pūtaiao hou, ko te hikohiko pūmua te tikanga matua mō te tātari me te whakaahua i ngā pūmua. Kei te ngako o tēnei tikanga tePolyacrylamide, he matū maha-whakamahinga e mahi ana hei pou tuara o ngā matrices gel e whakamahia ana i roto i ngā pūnaha electrophoresis gel. Nā ngā āhuatanga ahurei o te Polyacrylamide he taputapu tino nui mō ngā kairangahau me ngā kaipūtaiao e rapu ana ki te wetewete i ngā uauatanga o ngā pūmua me ā rātou taunekeneke.

Ko te Polyacrylamide, e mōhiotia whānuitia ana ko te PAM, he pūmua hangai i hangaia mai i ngā monomer acrylamide. Ko tōna whānuitanga whakamiharo e pā ana ki tōna kaha ki te hanga mekameka roa, ka hua ake he matū rite ki te tiēre ka taea te whakauru i ngā rahi rerekē o ngā ngota. Nā tēnei āhuatanga ka waiho te polyacrylamide hei kaitono pai mō te hanga i ngā matrices porous e whakamahia ana i roto i te electrophoresis pūmua.

Ko te hikohiko pūmua he tikanga e wehewehe ana i ngā pūmua i runga i tō rātou utu me te rahi. Mā te tuku i tētahi tauira pūmua ki tētahi mara hiko i roto i tētahi matrix polyacrylamide gel, ka heke ngā pūmua i roto i te gel i ngā tere rerekē, ka hua ake he roopu motuhake ka taea te tātari me te ine. Mā tēnei wehenga ka whakaratohia he māramatanga nui ki te parakore o te pūmua, te whakatau i te taumaha ngota, me te aroaro o ngā isoforms.

Te Tūranga o te Polyacrylamide i roto i te Pūmua Hikohiko

Ko te kōwhiringa o te polyacrylamide mō te electrophoresis pūmua e ahu mai ana i tōna āhua ngāwari ki te whakarerekē. Ka taea e ngā kaipūtaiao te whakarerekē i te kukū o te matrix gel kia uru ki ngā pūmua o ngā rahi rerekē. Mā ngā kukū teitei ake ka hangaia he matrices kikī ake e tika ana mō te whakatau i ngā pūmua iti ake, ko ngā kukū iti ake ka whakamahia mō ngā pūmua nui ake. Mā tēnei urutau ka taea e ngā kairangahau te whakarite i ā rātou whakamātautau kia tutuki ai te wehenga me te tātaritanga tino pai.

Polyacrylamide heiKaiwhakarewa wai

Ko te whai huatanga o te Polyacrylamide kei tua atu i tana mahi i roto i te electrophoresis gel. Ka kitea hoki ngā tono hei flocculant i roto i ngā momo ahumahi, pērā i te maimoatanga wai me te whakahaere wai para. Hei flocculant, ka āwhina te polyacrylamide ki te whakakotahi i ngā matūriki iri i roto i ngā wai, ka āwhina i te tango atu. E whakaatu ana tēnei āhuatanga i ngā kaha kanorau o te matū me te whānuitanga o te pānga ki te pūtaiao me te ahumahi.

Ngā Whakatairanga i roto i te Electrophoresis e Hangai ana ki te Polyacrylamide

Kua kitea ngā whanaketanga tonu o ngā tikanga hikohiko e ahu mai ana i te polyacrylamide i ngā tau tata nei. Ko te Native PAGE, ko te SDS-PAGE, me te hikohiko gel rua-ahu he tauira torutoru noa iho o te āheinga o te polyacrylamide ki te whakawhanake i ngā tikanga motuhake mō te tātari i ngā hanganga pūmua, ngā whakarerekētanga i muri i te whakamaoritanga, me ngā taunekeneke. He mea tino nui ēnei tikanga i roto i te rangahau proteomics me ngā mahi kimi rongoā.

I roto i te ao o te tātari pūmua, ka puta te polyacrylamide hei hoa pumau, e āhei ai ngā kairangahau ki te ruku hohonu ki te ao uaua o ngā pūmua. Kāore e taea te whakanui i tōna tūranga hei turanga mō ngā matrices gel i roto i ngā pūnaha electrophoresis. Mai i te wetewete i ngā tikanga mate ki te whakawhanake i ngā rongoā hou, kei te hanga tonu te electrophoresis e hangai ana ki te polyacrylamide i te ahunga whakamua o te pūtaiao. I te whanaketanga o te hangarau, tera pea ka whanake tēnei mahi whakamiharo, ka whakarei ake i tō tātou māramatanga ki ngā pūmua me ā rātou mahi maha.